9/7/2023 0 Comments Endnote format for hhmi![]() The `frozen plasmon' method is introduced to explicitly model spatially variable inelastic scattering processes in cryo-EM specimens. ![]() Modeling both the signal and the noise accurately is necessary to simulate images of biological specimens with contrast that is correct on an absolute scale. simulations of a crowded or cellular environment. This practice fails for images that require spatially variable scaling, e.g. Current state-of-the-art simulators apply post hoc scaling to approximate empirical solvent contrast, attenuated image intensity due to specimen thickness and amplitude contrast. Simulating images with contrast that matches the contrast observed in experimental images remains challenging, especially for amorphous samples. Image simulation plays a central role in the development and practice of high-resolution electron microscopy, including transmission electron microscopy of frozen-hydrated specimens (cryo-EM).
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